PoplarV1, Main, Exploration, bibRecord, 000650

Transgenic Reduction of Cytokinin Levels in Roots Inhibits Root-Sprouting in Populus.

Identifieur interne : 000650 ( Main/Exploration ); précédent : 000649; suivant : 000651

Transgenic Reduction of Cytokinin Levels in Roots Inhibits Root-Sprouting in Populus.

Auteurs : Wei Li [États-Unis, République populaire de Chine] ; Longmei Zhai [États-Unis, République populaire de Chine] ; Steven H. Strauss [États-Unis] ; Huseyin Yer [États-Unis] ; Emily Merewitz [États-Unis] ; Junmei Chen [États-Unis] ; Xiaomin Wang [États-Unis, République populaire de Chine] ; Weibing Zhuang [États-Unis, République populaire de Chine] ; Chu Fang [États-Unis] ; Yingnan Chen [États-Unis] ; Richard Mcavoy [États-Unis] ; Zhenhai Han [République populaire de Chine] ; Yi Li [États-Unis]

Source :

Plant physiology [ 1532-2548 ] ; 2019.

RBID : pubmed:31152128

Descripteurs français

English descriptors

KwdEn :
- Cytokinins (metabolism), Gene Expression Regulation, Plant (genetics), Gene Expression Regulation, Plant (physiology), Plant Proteins (genetics), Plant Proteins (metabolism), Plants, Genetically Modified (genetics), Plants, Genetically Modified (metabolism), Populus (genetics), Populus (metabolism), Promoter Regions, Genetic (genetics).
MESH :
- chemical , genetics : Plant Proteins.
- chemical , metabolism : Cytokinins, Plant Proteins.
- genetics : Gene Expression Regulation, Plant, Plants, Genetically Modified, Populus, Promoter Regions, Genetic.
- metabolism : Plants, Genetically Modified, Populus.
- physiology : Gene Expression Regulation, Plant.

Abstract

Root sprouts-the formation of new shoots from roots-is an important mechanism for local gene flow from poplar (Populus spp). An effective strategy to reduce root sprout formation could therefore help to ensure containment during field research and commercial deployment of poplar when grown as exotic or transgenic forms. We used a flavonoid glycosyltransferase gene promoter from Scutellaria barbata (SbUGT) to drive the expression of AtCKX2, a cytokinin oxidase from Arabidopsis that converts active to inactive cytokinins in roots of poplar. In the greenhouse, SbUGT::AtCKX2 transgenic plants exhibited a similar shoot growth habit, but had enhanced root growth and fewer root sprouts, compared to the wild-type control and transgenic events with low transgene expression in roots. Under field conditions, the transgenic trees also had similar growth habits and stem growth rates that were not statistically different from wild-type trees over 3 years. Removal of trunks generally induced high rates of root sprouting; however, in selected SbUGT::AtCKX2 transgenic poplar events there was an absence or fewer root sprouts compared to wild-type trees, consistent with the greenhouse results. Our study demonstrates that the SbUGT::AtCKX2 gene can effectively inhibit root sprouting of poplar trees under field conditions, and thus may provide a useful tool to address concerns associated with root-sprouting-mediated transgene spread.

DOI: 10.1104/pp.19.00217
PubMed: 31152128
PubMed Central: PMC6670097

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000862
to stream Main, to step Curation: 000862

Le document en format XML

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<term>Gene Expression Regulation, Plant (genetics)</term>
<term>Gene Expression Regulation, Plant (physiology)</term>
<term>Plant Proteins (genetics)</term>
<term>Plant Proteins (metabolism)</term>
<term>Plants, Genetically Modified (genetics)</term>
<term>Plants, Genetically Modified (metabolism)</term>
<term>Populus (genetics)</term>
<term>Populus (metabolism)</term>
<term>Promoter Regions, Genetic (genetics)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Cytokinine (métabolisme)</term>
<term>Populus (génétique)</term>
<term>Populus (métabolisme)</term>
<term>Protéines végétales (génétique)</term>
<term>Protéines végétales (métabolisme)</term>
<term>Régions promotrices (génétique) (génétique)</term>
<term>Régulation de l'expression des gènes végétaux (génétique)</term>
<term>Régulation de l'expression des gènes végétaux (physiologie)</term>
<term>Végétaux génétiquement modifiés (génétique)</term>
<term>Végétaux génétiquement modifiés (métabolisme)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cytokinins</term>
<term>Plant Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Gene Expression Regulation, Plant</term>
<term>Plants, Genetically Modified</term>
<term>Populus</term>
<term>Promoter Regions, Genetic</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Populus</term>
<term>Protéines végétales</term>
<term>Régions promotrices (génétique)</term>
<term>Régulation de l'expression des gènes végétaux</term>
<term>Végétaux génétiquement modifiés</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plants, Genetically Modified</term>
<term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cytokinine</term>
<term>Populus</term>
<term>Protéines végétales</term>
<term>Végétaux génétiquement modifiés</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Régulation de l'expression des gènes végétaux</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Gene Expression Regulation, Plant</term>
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</teiHeader>
<front><div type="abstract" xml:lang="en">Root sprouts-the formation of new shoots from roots-is an important mechanism for local gene flow from poplar (<i>Populus</i>
 spp). An effective strategy to reduce root sprout formation could therefore help to ensure containment during field research and commercial deployment of poplar when grown as exotic or transgenic forms. We used a flavonoid glycosyltransferase gene promoter from <i>Scutellaria barbata</i>
 (<i>SbUGT</i>
) to drive the expression of <i>AtCKX2</i>
, a cytokinin oxidase from Arabidopsis that converts active to inactive cytokinins in roots of poplar. In the greenhouse, <i>SbUGT</i>
::<i>AtCKX2</i>
 transgenic plants exhibited a similar shoot growth habit, but had enhanced root growth and fewer root sprouts, compared to the wild-type control and transgenic events with low transgene expression in roots. Under field conditions, the transgenic trees also had similar growth habits and stem growth rates that were not statistically different from wild-type trees over 3 years. Removal of trunks generally induced high rates of root sprouting; however, in selected <i>SbUGT</i>
::<i>AtCKX2</i>
 transgenic poplar events there was an absence or fewer root sprouts compared to wild-type trees, consistent with the greenhouse results. Our study demonstrates that the <i>SbUGT</i>
::<i>AtCKX2</i>
 gene can effectively inhibit root sprouting of poplar trees under field conditions, and thus may provide a useful tool to address concerns associated with root-sprouting-mediated transgene spread.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31152128</PMID>
<DateCompleted><Year>2020</Year>
<Month>08</Month>
<Day>24</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>08</Month>
<Day>24</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1532-2548</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>180</Volume>
<Issue>4</Issue>
<PubDate><Year>2019</Year>
<Month>08</Month>
</PubDate>
</JournalIssue>
<Title>Plant physiology</Title>
<ISOAbbreviation>Plant Physiol</ISOAbbreviation>
</Journal>
<ArticleTitle>Transgenic Reduction of Cytokinin Levels in Roots Inhibits Root-Sprouting in <i>Populus</i>
.</ArticleTitle>
<Pagination><MedlinePgn>1788-1792</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1104/pp.19.00217</ELocationID>
<Abstract><AbstractText>Root sprouts-the formation of new shoots from roots-is an important mechanism for local gene flow from poplar (<i>Populus</i>
 spp). An effective strategy to reduce root sprout formation could therefore help to ensure containment during field research and commercial deployment of poplar when grown as exotic or transgenic forms. We used a flavonoid glycosyltransferase gene promoter from <i>Scutellaria barbata</i>
 (<i>SbUGT</i>
) to drive the expression of <i>AtCKX2</i>
, a cytokinin oxidase from Arabidopsis that converts active to inactive cytokinins in roots of poplar. In the greenhouse, <i>SbUGT</i>
::<i>AtCKX2</i>
 transgenic plants exhibited a similar shoot growth habit, but had enhanced root growth and fewer root sprouts, compared to the wild-type control and transgenic events with low transgene expression in roots. Under field conditions, the transgenic trees also had similar growth habits and stem growth rates that were not statistically different from wild-type trees over 3 years. Removal of trunks generally induced high rates of root sprouting; however, in selected <i>SbUGT</i>
::<i>AtCKX2</i>
 transgenic poplar events there was an absence or fewer root sprouts compared to wild-type trees, consistent with the greenhouse results. Our study demonstrates that the <i>SbUGT</i>
::<i>AtCKX2</i>
 gene can effectively inhibit root sprouting of poplar trees under field conditions, and thus may provide a useful tool to address concerns associated with root-sprouting-mediated transgene spread.</AbstractText>
<CopyrightInformation>© 2019 American Society of Plant Biologists. All Rights Reserved.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName>
<ForeName>Wei</ForeName>
<Initials>W</Initials>
<Identifier Source="ORCID">0000-0002-7989-1189</Identifier>
<AffiliationInfo><Affiliation>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, Connecticut 06269.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>College of Horticulture, China Agricultural University, Beijing 100193, P. R. China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhai</LastName>
<ForeName>Longmei</ForeName>
<Initials>L</Initials>
<Identifier Source="ORCID">0000-0003-4199-5625</Identifier>
<AffiliationInfo><Affiliation>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, Connecticut 06269.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>College of Horticulture, China Agricultural University, Beijing 100193, P. R. China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Strauss</LastName>
<ForeName>Steven H</ForeName>
<Initials>SH</Initials>
<AffiliationInfo><Affiliation>Department of Forest Ecosystems and Society, Oregon State University, Corvallis, Oregon 97331.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Yer</LastName>
<ForeName>Huseyin</ForeName>
<Initials>H</Initials>
<AffiliationInfo><Affiliation>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, Connecticut 06269.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Merewitz</LastName>
<ForeName>Emily</ForeName>
<Initials>E</Initials>
<AffiliationInfo><Affiliation>Department of Plant Soil and Microbial Sciences, Michigan State University, East Lansing, Michigan 48824.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chen</LastName>
<ForeName>Junmei</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, Connecticut 06269.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Xiaomin</ForeName>
<Initials>X</Initials>
<AffiliationInfo><Affiliation>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, Connecticut 06269.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, P. R. China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhuang</LastName>
<ForeName>Weibing</ForeName>
<Initials>W</Initials>
<AffiliationInfo><Affiliation>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, Connecticut 06269.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, P. R. China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Fang</LastName>
<ForeName>Chu</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, Connecticut 06269.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chen</LastName>
<ForeName>Yingnan</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, Connecticut 06269.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>McAvoy</LastName>
<ForeName>Richard</ForeName>
<Initials>R</Initials>
<AffiliationInfo><Affiliation>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, Connecticut 06269.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Han</LastName>
<ForeName>Zhenhai</ForeName>
<Initials>Z</Initials>
<Identifier Source="ORCID">0000-0003-3759-1046</Identifier>
<AffiliationInfo><Affiliation>College of Horticulture, China Agricultural University, Beijing 100193, P. R. China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Li</LastName>
<ForeName>Yi</ForeName>
<Initials>Y</Initials>
<Identifier Source="ORCID">0000-0001-5106-8783</Identifier>
<AffiliationInfo><Affiliation>Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, Connecticut 06269 yi.li@uconn.edu.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016422">Letter</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
<PublicationType UI="D013486">Research Support, U.S. Gov't, Non-P.H.S.</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2019</Year>
<Month>05</Month>
<Day>31</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>Plant Physiol</MedlineTA>
<NlmUniqueID>0401224</NlmUniqueID>
<ISSNLinking>0032-0889</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D003583">Cytokinins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D003583" MajorTopicYN="N">Cytokinins</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011401" MajorTopicYN="N">Promoter Regions, Genetic</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year>
<Month>02</Month>
<Day>21</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2019</Year>
<Month>05</Month>
<Day>19</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2019</Year>
<Month>6</Month>
<Day>4</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2020</Year>
<Month>8</Month>
<Day>25</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2019</Year>
<Month>6</Month>
<Day>2</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
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<ArticleId IdType="pii">pp.19.00217</ArticleId>
<ArticleId IdType="doi">10.1104/pp.19.00217</ArticleId>
<ArticleId IdType="pmc">PMC6670097</ArticleId>
</ArticleIdList>
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</pubmed>
<affiliations><list><country><li>République populaire de Chine</li>
<li>États-Unis</li>
</country>
<region><li>Connecticut</li>
<li>Michigan</li>
<li>Oregon</li>
</region>
<settlement><li>Pékin</li>
</settlement>
</list>
<tree><country name="États-Unis"><region name="Connecticut"><name sortKey="Li, Wei" sort="Li, Wei" uniqKey="Li W" first="Wei" last="Li">Wei Li</name>
</region>
<name sortKey="Chen, Junmei" sort="Chen, Junmei" uniqKey="Chen J" first="Junmei" last="Chen">Junmei Chen</name>
<name sortKey="Chen, Yingnan" sort="Chen, Yingnan" uniqKey="Chen Y" first="Yingnan" last="Chen">Yingnan Chen</name>
<name sortKey="Fang, Chu" sort="Fang, Chu" uniqKey="Fang C" first="Chu" last="Fang">Chu Fang</name>
<name sortKey="Li, Yi" sort="Li, Yi" uniqKey="Li Y" first="Yi" last="Li">Yi Li</name>
<name sortKey="Mcavoy, Richard" sort="Mcavoy, Richard" uniqKey="Mcavoy R" first="Richard" last="Mcavoy">Richard Mcavoy</name>
<name sortKey="Merewitz, Emily" sort="Merewitz, Emily" uniqKey="Merewitz E" first="Emily" last="Merewitz">Emily Merewitz</name>
<name sortKey="Strauss, Steven H" sort="Strauss, Steven H" uniqKey="Strauss S" first="Steven H" last="Strauss">Steven H. Strauss</name>
<name sortKey="Wang, Xiaomin" sort="Wang, Xiaomin" uniqKey="Wang X" first="Xiaomin" last="Wang">Xiaomin Wang</name>
<name sortKey="Yer, Huseyin" sort="Yer, Huseyin" uniqKey="Yer H" first="Huseyin" last="Yer">Huseyin Yer</name>
<name sortKey="Zhai, Longmei" sort="Zhai, Longmei" uniqKey="Zhai L" first="Longmei" last="Zhai">Longmei Zhai</name>
<name sortKey="Zhuang, Weibing" sort="Zhuang, Weibing" uniqKey="Zhuang W" first="Weibing" last="Zhuang">Weibing Zhuang</name>
</country>
<country name="République populaire de Chine"><noRegion><name sortKey="Li, Wei" sort="Li, Wei" uniqKey="Li W" first="Wei" last="Li">Wei Li</name>
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<name sortKey="Wang, Xiaomin" sort="Wang, Xiaomin" uniqKey="Wang X" first="Xiaomin" last="Wang">Xiaomin Wang</name>
<name sortKey="Zhai, Longmei" sort="Zhai, Longmei" uniqKey="Zhai L" first="Longmei" last="Zhai">Longmei Zhai</name>
<name sortKey="Zhuang, Weibing" sort="Zhuang, Weibing" uniqKey="Zhuang W" first="Weibing" last="Zhuang">Weibing Zhuang</name>
</country>
</tree>
</affiliations>
</record>

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Transgenic Reduction of Cytokinin Levels in Roots Inhibits Root-Sprouting in Populus.

Transgenic Reduction of Cytokinin Levels in Roots Inhibits Root-Sprouting in Populus.

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